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KMID : 0613820180280010001
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Journal of Life Science
2018 Volume.28 No. 1 p.1 ~ p.8
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Metabolism of Lactate Dehydrogenase in Tissues from Ldh-C Expressed Mice (Mus musculus) in a Starvation State
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Yum Jung-Joo
Kim Gyu-Dong
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Abstract
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To confirm the function of lactate dehydrogenase (LDH) (EC 1.1.1.27, LDH), its metabolism was studied by activity, kinetics, and isozyme analysis in tissues of Ldh testis-specific C expressing mice (Mus musculus) maintained in a state of starvation for 48 hr and 96 hr. In skeletal muscle, liver, and eye tissues, LDH and LDH A4 activity increased and anaerobic metabolism predominated. While LDH activity in the heart and kidney tissues decreased, LDH B4 activity increased and aerobic metabolism predominated, producing pyruvic acid. In the testis tissue, LDH C4 activity decreased. In the brain tissue, LDH activity increased, but the isozyme change was small and the amount of pyruvic acid decreased. KmPYR increased in tissues other than kidney tissue, and the affinity for pyruvic acid decreased. Consequently, in Ldh-A and B-expressing tissues, the activities of isozymes with higher concentrations increased. However, in Ldh-A, B, and C-expressing tissue, C4 decreased and the function of the tissue also decreased. In particular, LDH in brain tissue played a role as a pyruvate reductase. Therefore, this process might be the mechanism for producing energy in the state of starvation.
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KEYWORD
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mPYR, lactate dehydrogenase, LDH testis-specific C isozyme, Mus musculus, starvation
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